Voyager DP-263-S Guide de l'utilisateur télécharger pdf (Page 304)

100

101

Chapter 5 Optimizing Instrument Settings

5-74 PerSeptive Biosystems

If the resolution does not improve, decrease the Grid

Voltage% (by 0.5 percent for Linear mode, or 1.0 percent

for Reflector mode), acquire a new spectrum and observe.

4. Continue increasing (or decreasing) the Grid Voltage% in

increments (or decrements) of 0.5 percent (Linear mode)

or 1.0 percent (Reflector mode) until optimum resolution is

obtained.

Hint: If you obtain close to acceptable resolution at one

setting, but less acceptable resolution at the next

setting, you may have adjusted the Grid Voltage% too

far. Increase or decrease in small increments until you

obtain optimum resolution.

5. Adjust the Guide Wire Voltage as needed:

• Increase the setting to increase sensitivity for

higher masses.

• For Voyager-DE, Voyager-DE PRO, and

Voyager-DE STR models in Reflector mode, adjust

the Guide Wire between 0 and 0.04 percent.

6. If you are analyzing peptides below 2,000 Da, you may be

able to improve resolution by adjusting the Accelerating

Voltage:

• Linear mode—Decrease from 20,000 V to

15,000 V to increase flight times.

• Reflector mode—Decrease to 15,000 V,

10,000 V, or 5,000 V. However, these lower

settings will compromise sensitivity.

7. Save the .BIC file.

1 2 ... 299 300 301 302 303 304 305 306 307 308 309 ... 611 612

Commentaires sur ces manuels

Pas de commentaire

Voyager DP-263-S Guide de l'utilisateur Page 304

Commentaires sur ces manuels

Produits connexes et manuels pour Interphones de porte Voyager DP-263-S